cfx 96 touch qpcr instrument 182 Search Results


mir 183 family  (ATCC)
92
ATCC mir 183 family
Mir 183 Family, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mir 183 family/product/ATCC
Average 92 stars, based on 1 article reviews
mir 183 family - by Bioz Stars, 2026-03
92/100 stars
  Buy from Supplier
lightcycler480 ii system  (Roche)
99
Roche lightcycler480 ii system
Lightcycler480 Ii System, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lightcycler480 ii system/product/Roche
Average 99 stars, based on 1 article reviews
lightcycler480 ii system - by Bioz Stars, 2026-03
99/100 stars
  Buy from Supplier
96-well deep well plates  (Greiner Bio)
90
Greiner Bio 96-well deep well plates
96 Well Deep Well Plates, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/96-well deep well plates/product/Greiner Bio
Average 90 stars, based on 1 article reviews
96-well deep well plates - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
mir-183  (Thermo Fisher)
90
Thermo Fisher mir-183
In vitro inhibition of miR-182 alone and <t>miR-183C</t> <t>miRNAs</t> in splenocytes reduces inflammatory cytokines IFNγ and IL-6 expression level. (A) Antagomir-182, -96, -183 inhibited the respective miRNA efficiently and specifically. Freshly-isolated splenocytes from MRL/lpr mice were transfected with either scramble control, antagomir-182, or the mixture of antagomir-182, -96, and -183 (antagomir-183C). Twenty-four hours after transfection, the cells were collected for TaqMan miRNA assays. The graph showed means ± SD ( n = 2 each). (B,C) Inhibition of miR-182 or miR-183C reduced IFNγ and IL-6 production in LPS or Con A-activated splenocytes. Freshly-isolated splenocytes from MRL and autoimmune-prone MRL/lpr mice at 14–15 weeks of age were treated with control antagomir and specific antagomir for 24 h, and then stimulated with LPS (500 ng/ml) for 48 h or Con A (5 µg/ml) for 24 h. The IFNγ and IL-6 levels in the culture supernatant were measured by ELISA. The graphs showed means ± SD ( n ≥ 6). The cytokine level in specific antagomir treated samples were shown as the percentage of paired scrambled control antagomir treated cells. Paired student t tests were performed (scrambled control vs . antagomir-182 or antagomir-183C); **, p < 0.01; and ***, p < 0.001.
Mir 183, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mir-183/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
mir-183 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
immunosorbent assay kit  (Aviva Systems)
92
Aviva Systems immunosorbent assay kit
In vitro inhibition of miR-182 alone and <t>miR-183C</t> <t>miRNAs</t> in splenocytes reduces inflammatory cytokines IFNγ and IL-6 expression level. (A) Antagomir-182, -96, -183 inhibited the respective miRNA efficiently and specifically. Freshly-isolated splenocytes from MRL/lpr mice were transfected with either scramble control, antagomir-182, or the mixture of antagomir-182, -96, and -183 (antagomir-183C). Twenty-four hours after transfection, the cells were collected for TaqMan miRNA assays. The graph showed means ± SD ( n = 2 each). (B,C) Inhibition of miR-182 or miR-183C reduced IFNγ and IL-6 production in LPS or Con A-activated splenocytes. Freshly-isolated splenocytes from MRL and autoimmune-prone MRL/lpr mice at 14–15 weeks of age were treated with control antagomir and specific antagomir for 24 h, and then stimulated with LPS (500 ng/ml) for 48 h or Con A (5 µg/ml) for 24 h. The IFNγ and IL-6 levels in the culture supernatant were measured by ELISA. The graphs showed means ± SD ( n ≥ 6). The cytokine level in specific antagomir treated samples were shown as the percentage of paired scrambled control antagomir treated cells. Paired student t tests were performed (scrambled control vs . antagomir-182 or antagomir-183C); **, p < 0.01; and ***, p < 0.001.
Immunosorbent Assay Kit, supplied by Aviva Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/immunosorbent assay kit/product/Aviva Systems
Average 92 stars, based on 1 article reviews
immunosorbent assay kit - by Bioz Stars, 2026-03
92/100 stars
  Buy from Supplier
96-well flat-bottom plates immobilon-p  (Millipore)
90
Millipore 96-well flat-bottom plates immobilon-p
In vitro inhibition of miR-182 alone and <t>miR-183C</t> <t>miRNAs</t> in splenocytes reduces inflammatory cytokines IFNγ and IL-6 expression level. (A) Antagomir-182, -96, -183 inhibited the respective miRNA efficiently and specifically. Freshly-isolated splenocytes from MRL/lpr mice were transfected with either scramble control, antagomir-182, or the mixture of antagomir-182, -96, and -183 (antagomir-183C). Twenty-four hours after transfection, the cells were collected for TaqMan miRNA assays. The graph showed means ± SD ( n = 2 each). (B,C) Inhibition of miR-182 or miR-183C reduced IFNγ and IL-6 production in LPS or Con A-activated splenocytes. Freshly-isolated splenocytes from MRL and autoimmune-prone MRL/lpr mice at 14–15 weeks of age were treated with control antagomir and specific antagomir for 24 h, and then stimulated with LPS (500 ng/ml) for 48 h or Con A (5 µg/ml) for 24 h. The IFNγ and IL-6 levels in the culture supernatant were measured by ELISA. The graphs showed means ± SD ( n ≥ 6). The cytokine level in specific antagomir treated samples were shown as the percentage of paired scrambled control antagomir treated cells. Paired student t tests were performed (scrambled control vs . antagomir-182 or antagomir-183C); **, p < 0.01; and ***, p < 0.001.
96 Well Flat Bottom Plates Immobilon P, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/96-well flat-bottom plates immobilon-p/product/Millipore
Average 90 stars, based on 1 article reviews
96-well flat-bottom plates immobilon-p - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
cfx 96 touch qpcr instrument 182  (Bio-Rad)
93
Bio-Rad cfx 96 touch qpcr instrument 182
In vitro inhibition of miR-182 alone and <t>miR-183C</t> <t>miRNAs</t> in splenocytes reduces inflammatory cytokines IFNγ and IL-6 expression level. (A) Antagomir-182, -96, -183 inhibited the respective miRNA efficiently and specifically. Freshly-isolated splenocytes from MRL/lpr mice were transfected with either scramble control, antagomir-182, or the mixture of antagomir-182, -96, and -183 (antagomir-183C). Twenty-four hours after transfection, the cells were collected for TaqMan miRNA assays. The graph showed means ± SD ( n = 2 each). (B,C) Inhibition of miR-182 or miR-183C reduced IFNγ and IL-6 production in LPS or Con A-activated splenocytes. Freshly-isolated splenocytes from MRL and autoimmune-prone MRL/lpr mice at 14–15 weeks of age were treated with control antagomir and specific antagomir for 24 h, and then stimulated with LPS (500 ng/ml) for 48 h or Con A (5 µg/ml) for 24 h. The IFNγ and IL-6 levels in the culture supernatant were measured by ELISA. The graphs showed means ± SD ( n ≥ 6). The cytokine level in specific antagomir treated samples were shown as the percentage of paired scrambled control antagomir treated cells. Paired student t tests were performed (scrambled control vs . antagomir-182 or antagomir-183C); **, p < 0.01; and ***, p < 0.001.
Cfx 96 Touch Qpcr Instrument 182, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cfx 96 touch qpcr instrument 182/product/Bio-Rad
Average 93 stars, based on 1 article reviews
cfx 96 touch qpcr instrument 182 - by Bioz Stars, 2026-03
93/100 stars
  Buy from Supplier
black-wall 96-well microtiter plate  (Thermo Fisher)
90
Thermo Fisher black-wall 96-well microtiter plate
In vitro inhibition of miR-182 alone and <t>miR-183C</t> <t>miRNAs</t> in splenocytes reduces inflammatory cytokines IFNγ and IL-6 expression level. (A) Antagomir-182, -96, -183 inhibited the respective miRNA efficiently and specifically. Freshly-isolated splenocytes from MRL/lpr mice were transfected with either scramble control, antagomir-182, or the mixture of antagomir-182, -96, and -183 (antagomir-183C). Twenty-four hours after transfection, the cells were collected for TaqMan miRNA assays. The graph showed means ± SD ( n = 2 each). (B,C) Inhibition of miR-182 or miR-183C reduced IFNγ and IL-6 production in LPS or Con A-activated splenocytes. Freshly-isolated splenocytes from MRL and autoimmune-prone MRL/lpr mice at 14–15 weeks of age were treated with control antagomir and specific antagomir for 24 h, and then stimulated with LPS (500 ng/ml) for 48 h or Con A (5 µg/ml) for 24 h. The IFNγ and IL-6 levels in the culture supernatant were measured by ELISA. The graphs showed means ± SD ( n ≥ 6). The cytokine level in specific antagomir treated samples were shown as the percentage of paired scrambled control antagomir treated cells. Paired student t tests were performed (scrambled control vs . antagomir-182 or antagomir-183C); **, p < 0.01; and ***, p < 0.001.
Black Wall 96 Well Microtiter Plate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/black-wall 96-well microtiter plate/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
black-wall 96-well microtiter plate - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
primus 96 advanced thermal cycler  (PEQLAB)
90
PEQLAB primus 96 advanced thermal cycler
In vitro inhibition of miR-182 alone and <t>miR-183C</t> <t>miRNAs</t> in splenocytes reduces inflammatory cytokines IFNγ and IL-6 expression level. (A) Antagomir-182, -96, -183 inhibited the respective miRNA efficiently and specifically. Freshly-isolated splenocytes from MRL/lpr mice were transfected with either scramble control, antagomir-182, or the mixture of antagomir-182, -96, and -183 (antagomir-183C). Twenty-four hours after transfection, the cells were collected for TaqMan miRNA assays. The graph showed means ± SD ( n = 2 each). (B,C) Inhibition of miR-182 or miR-183C reduced IFNγ and IL-6 production in LPS or Con A-activated splenocytes. Freshly-isolated splenocytes from MRL and autoimmune-prone MRL/lpr mice at 14–15 weeks of age were treated with control antagomir and specific antagomir for 24 h, and then stimulated with LPS (500 ng/ml) for 48 h or Con A (5 µg/ml) for 24 h. The IFNγ and IL-6 levels in the culture supernatant were measured by ELISA. The graphs showed means ± SD ( n ≥ 6). The cytokine level in specific antagomir treated samples were shown as the percentage of paired scrambled control antagomir treated cells. Paired student t tests were performed (scrambled control vs . antagomir-182 or antagomir-183C); **, p < 0.01; and ***, p < 0.001.
Primus 96 Advanced Thermal Cycler, supplied by PEQLAB, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primus 96 advanced thermal cycler/product/PEQLAB
Average 90 stars, based on 1 article reviews
primus 96 advanced thermal cycler - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
mmu482690_mir  (Thermo Fisher)
90
Thermo Fisher mmu482690_mir
In vitro inhibition of miR-182 alone and <t>miR-183C</t> <t>miRNAs</t> in splenocytes reduces inflammatory cytokines IFNγ and IL-6 expression level. (A) Antagomir-182, -96, -183 inhibited the respective miRNA efficiently and specifically. Freshly-isolated splenocytes from MRL/lpr mice were transfected with either scramble control, antagomir-182, or the mixture of antagomir-182, -96, and -183 (antagomir-183C). Twenty-four hours after transfection, the cells were collected for TaqMan miRNA assays. The graph showed means ± SD ( n = 2 each). (B,C) Inhibition of miR-182 or miR-183C reduced IFNγ and IL-6 production in LPS or Con A-activated splenocytes. Freshly-isolated splenocytes from MRL and autoimmune-prone MRL/lpr mice at 14–15 weeks of age were treated with control antagomir and specific antagomir for 24 h, and then stimulated with LPS (500 ng/ml) for 48 h or Con A (5 µg/ml) for 24 h. The IFNγ and IL-6 levels in the culture supernatant were measured by ELISA. The graphs showed means ± SD ( n ≥ 6). The cytokine level in specific antagomir treated samples were shown as the percentage of paired scrambled control antagomir treated cells. Paired student t tests were performed (scrambled control vs . antagomir-182 or antagomir-183C); **, p < 0.01; and ***, p < 0.001.
Mmu482690 Mir, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mmu482690_mir/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
mmu482690_mir - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
96 well plates  (MedChemExpress)
94
MedChemExpress 96 well plates
In vitro inhibition of miR-182 alone and <t>miR-183C</t> <t>miRNAs</t> in splenocytes reduces inflammatory cytokines IFNγ and IL-6 expression level. (A) Antagomir-182, -96, -183 inhibited the respective miRNA efficiently and specifically. Freshly-isolated splenocytes from MRL/lpr mice were transfected with either scramble control, antagomir-182, or the mixture of antagomir-182, -96, and -183 (antagomir-183C). Twenty-four hours after transfection, the cells were collected for TaqMan miRNA assays. The graph showed means ± SD ( n = 2 each). (B,C) Inhibition of miR-182 or miR-183C reduced IFNγ and IL-6 production in LPS or Con A-activated splenocytes. Freshly-isolated splenocytes from MRL and autoimmune-prone MRL/lpr mice at 14–15 weeks of age were treated with control antagomir and specific antagomir for 24 h, and then stimulated with LPS (500 ng/ml) for 48 h or Con A (5 µg/ml) for 24 h. The IFNγ and IL-6 levels in the culture supernatant were measured by ELISA. The graphs showed means ± SD ( n ≥ 6). The cytokine level in specific antagomir treated samples were shown as the percentage of paired scrambled control antagomir treated cells. Paired student t tests were performed (scrambled control vs . antagomir-182 or antagomir-183C); **, p < 0.01; and ***, p < 0.001.
96 Well Plates, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/96 well plates/product/MedChemExpress
Average 94 stars, based on 1 article reviews
96 well plates - by Bioz Stars, 2026-03
94/100 stars
  Buy from Supplier
mts/pms substrate celltiter 96® aqueous non-radioactive cell proliferation assay  (Promega)
90
Promega mts/pms substrate celltiter 96® aqueous non-radioactive cell proliferation assay
In vitro inhibition of miR-182 alone and <t>miR-183C</t> <t>miRNAs</t> in splenocytes reduces inflammatory cytokines IFNγ and IL-6 expression level. (A) Antagomir-182, -96, -183 inhibited the respective miRNA efficiently and specifically. Freshly-isolated splenocytes from MRL/lpr mice were transfected with either scramble control, antagomir-182, or the mixture of antagomir-182, -96, and -183 (antagomir-183C). Twenty-four hours after transfection, the cells were collected for TaqMan miRNA assays. The graph showed means ± SD ( n = 2 each). (B,C) Inhibition of miR-182 or miR-183C reduced IFNγ and IL-6 production in LPS or Con A-activated splenocytes. Freshly-isolated splenocytes from MRL and autoimmune-prone MRL/lpr mice at 14–15 weeks of age were treated with control antagomir and specific antagomir for 24 h, and then stimulated with LPS (500 ng/ml) for 48 h or Con A (5 µg/ml) for 24 h. The IFNγ and IL-6 levels in the culture supernatant were measured by ELISA. The graphs showed means ± SD ( n ≥ 6). The cytokine level in specific antagomir treated samples were shown as the percentage of paired scrambled control antagomir treated cells. Paired student t tests were performed (scrambled control vs . antagomir-182 or antagomir-183C); **, p < 0.01; and ***, p < 0.001.
Mts/Pms Substrate Celltiter 96® Aqueous Non Radioactive Cell Proliferation Assay, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mts/pms substrate celltiter 96® aqueous non-radioactive cell proliferation assay/product/Promega
Average 90 stars, based on 1 article reviews
mts/pms substrate celltiter 96® aqueous non-radioactive cell proliferation assay - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

Image Search Results


In vitro inhibition of miR-182 alone and miR-183C miRNAs in splenocytes reduces inflammatory cytokines IFNγ and IL-6 expression level. (A) Antagomir-182, -96, -183 inhibited the respective miRNA efficiently and specifically. Freshly-isolated splenocytes from MRL/lpr mice were transfected with either scramble control, antagomir-182, or the mixture of antagomir-182, -96, and -183 (antagomir-183C). Twenty-four hours after transfection, the cells were collected for TaqMan miRNA assays. The graph showed means ± SD ( n = 2 each). (B,C) Inhibition of miR-182 or miR-183C reduced IFNγ and IL-6 production in LPS or Con A-activated splenocytes. Freshly-isolated splenocytes from MRL and autoimmune-prone MRL/lpr mice at 14–15 weeks of age were treated with control antagomir and specific antagomir for 24 h, and then stimulated with LPS (500 ng/ml) for 48 h or Con A (5 µg/ml) for 24 h. The IFNγ and IL-6 levels in the culture supernatant were measured by ELISA. The graphs showed means ± SD ( n ≥ 6). The cytokine level in specific antagomir treated samples were shown as the percentage of paired scrambled control antagomir treated cells. Paired student t tests were performed (scrambled control vs . antagomir-182 or antagomir-183C); **, p < 0.01; and ***, p < 0.001.

Journal: Frontiers in Genetics

Article Title: Deletion of microRNA-183-96-182 Cluster in Lymphocytes Suppresses Anti-DsDNA Autoantibody Production and IgG Deposition in the Kidneys in C57BL/6-Fas lpr/lpr Mice

doi: 10.3389/fgene.2022.840060

Figure Lengend Snippet: In vitro inhibition of miR-182 alone and miR-183C miRNAs in splenocytes reduces inflammatory cytokines IFNγ and IL-6 expression level. (A) Antagomir-182, -96, -183 inhibited the respective miRNA efficiently and specifically. Freshly-isolated splenocytes from MRL/lpr mice were transfected with either scramble control, antagomir-182, or the mixture of antagomir-182, -96, and -183 (antagomir-183C). Twenty-four hours after transfection, the cells were collected for TaqMan miRNA assays. The graph showed means ± SD ( n = 2 each). (B,C) Inhibition of miR-182 or miR-183C reduced IFNγ and IL-6 production in LPS or Con A-activated splenocytes. Freshly-isolated splenocytes from MRL and autoimmune-prone MRL/lpr mice at 14–15 weeks of age were treated with control antagomir and specific antagomir for 24 h, and then stimulated with LPS (500 ng/ml) for 48 h or Con A (5 µg/ml) for 24 h. The IFNγ and IL-6 levels in the culture supernatant were measured by ELISA. The graphs showed means ± SD ( n ≥ 6). The cytokine level in specific antagomir treated samples were shown as the percentage of paired scrambled control antagomir treated cells. Paired student t tests were performed (scrambled control vs . antagomir-182 or antagomir-183C); **, p < 0.01; and ***, p < 0.001.

Article Snippet: As we previously reported ( ; ; ; ), TaqMan microRNA assays (Applied Biosystems, Waltham, MA, United States) were used to quantify miR-183C miRNAs (miR-182 ID 002599; miR-183 ID 00269; miR-96 ID 000186; snoRNA202 ID 001232, Applied Biosystems) expression levels according to the manufacturer’s instruction.

Techniques: In Vitro, Inhibition, Expressing, Isolation, Transfection, Enzyme-linked Immunosorbent Assay

Analysis of the effect of miR-183C deletion on body weight, spleen weight, and antibody production. (A) miRNA TaqMan assay was performed to validate the deletion of miR-182, -96, -183 miRNAs in purified CD4 + T and CD19 + B cells from conditional miR-183C −/− B6/lpr (miR-183C −/− ) mice. Data are presented as means ± SD ( n = 2 each). (B–E) Deletion of miR-183C had no obvious effect on body weight (B) , spleen weight (C) , spleen/body weight ratio (D) , and splenocyte counts (E) . The miR-183C −/− and control miR-183C fl/fl B6/lpr (miR-183C fl/fl ) mice were euthanized at 29–32 weeks of age (end point) for the assay. Data are displayed as means ± SD ( n ≥ 10). (F) Deletion of miR-183C miRNAs significantly reduced serum anti-dsDNA autoantibodies. The serum anti-dsDNA autoantibody levels in miR-183C −/− and control miR-183C fl/fl mice were monitored every 4 weeks starting at 20 weeks of age. The graph showed means ± SD ( n = 6 for miR-183C −/− mice at different time points except n = 12 for the end point; n = 10 for 183C fl/fl mice at different time points except n = 15 for the end point). (G,H) Evaluation of serum levels of total IgG (G) and IgM (H) antibodies in miR-183C −/− and control mice at the end point. Graphs showed means ± SD ( n = 10 each). Unpaired student t tests were performed (miR-183C fl/fl vs . miR-183C −/− ); *, p < 0.05; **, p < 0.01.

Journal: Frontiers in Genetics

Article Title: Deletion of microRNA-183-96-182 Cluster in Lymphocytes Suppresses Anti-DsDNA Autoantibody Production and IgG Deposition in the Kidneys in C57BL/6-Fas lpr/lpr Mice

doi: 10.3389/fgene.2022.840060

Figure Lengend Snippet: Analysis of the effect of miR-183C deletion on body weight, spleen weight, and antibody production. (A) miRNA TaqMan assay was performed to validate the deletion of miR-182, -96, -183 miRNAs in purified CD4 + T and CD19 + B cells from conditional miR-183C −/− B6/lpr (miR-183C −/− ) mice. Data are presented as means ± SD ( n = 2 each). (B–E) Deletion of miR-183C had no obvious effect on body weight (B) , spleen weight (C) , spleen/body weight ratio (D) , and splenocyte counts (E) . The miR-183C −/− and control miR-183C fl/fl B6/lpr (miR-183C fl/fl ) mice were euthanized at 29–32 weeks of age (end point) for the assay. Data are displayed as means ± SD ( n ≥ 10). (F) Deletion of miR-183C miRNAs significantly reduced serum anti-dsDNA autoantibodies. The serum anti-dsDNA autoantibody levels in miR-183C −/− and control miR-183C fl/fl mice were monitored every 4 weeks starting at 20 weeks of age. The graph showed means ± SD ( n = 6 for miR-183C −/− mice at different time points except n = 12 for the end point; n = 10 for 183C fl/fl mice at different time points except n = 15 for the end point). (G,H) Evaluation of serum levels of total IgG (G) and IgM (H) antibodies in miR-183C −/− and control mice at the end point. Graphs showed means ± SD ( n = 10 each). Unpaired student t tests were performed (miR-183C fl/fl vs . miR-183C −/− ); *, p < 0.05; **, p < 0.01.

Article Snippet: As we previously reported ( ; ; ; ), TaqMan microRNA assays (Applied Biosystems, Waltham, MA, United States) were used to quantify miR-183C miRNAs (miR-182 ID 002599; miR-183 ID 00269; miR-96 ID 000186; snoRNA202 ID 001232, Applied Biosystems) expression levels according to the manufacturer’s instruction.

Techniques: TaqMan Assay, Purification

Deletion of either miR-183C or miR-182 alone reduces IgG antibody deposition in kidney. (A,B) Summary graphs show no significant difference in the proteinuria (A) and blood urea nitrogen (BUN, (B) levels between miR-183C −/− B6/lpr and control miR-183C fl/fl B6/lpr mice. Graphs showed means ± SD ( n ≥ 5) (C,D) Representing IF images and summary data show significantly reduced IgG (C) and complement C3 (D) immune complex deposition in the kidneys of miR-183C −/− . Graphs showed means ± SD ( n = 10 each) (E,F) Summary graphs show no significant difference in the proteinuria (E) and blood urea nitrogen (BUN, (F) ) levels between miR-182 −/− B6/lpr and control miR-182 fl/fl B6/lpr mice. Graphs showed means ± SD ( n ≥ 5) (G,H) Representing images and summary data show significantly reduced IgG (G) and complement C3 (H) immune complex deposition in the kidneys of miR-182 −/− . Graphs showed means ± SD ( n = 10 each). Unpaired student t tests were performed (miR-183C fl/fl vs . miR-183C −/- , miR-182 fl/fl vs . miR-182 −/− ); *, p < 0.05; and **, p < 0.01.

Journal: Frontiers in Genetics

Article Title: Deletion of microRNA-183-96-182 Cluster in Lymphocytes Suppresses Anti-DsDNA Autoantibody Production and IgG Deposition in the Kidneys in C57BL/6-Fas lpr/lpr Mice

doi: 10.3389/fgene.2022.840060

Figure Lengend Snippet: Deletion of either miR-183C or miR-182 alone reduces IgG antibody deposition in kidney. (A,B) Summary graphs show no significant difference in the proteinuria (A) and blood urea nitrogen (BUN, (B) levels between miR-183C −/− B6/lpr and control miR-183C fl/fl B6/lpr mice. Graphs showed means ± SD ( n ≥ 5) (C,D) Representing IF images and summary data show significantly reduced IgG (C) and complement C3 (D) immune complex deposition in the kidneys of miR-183C −/− . Graphs showed means ± SD ( n = 10 each) (E,F) Summary graphs show no significant difference in the proteinuria (E) and blood urea nitrogen (BUN, (F) ) levels between miR-182 −/− B6/lpr and control miR-182 fl/fl B6/lpr mice. Graphs showed means ± SD ( n ≥ 5) (G,H) Representing images and summary data show significantly reduced IgG (G) and complement C3 (H) immune complex deposition in the kidneys of miR-182 −/− . Graphs showed means ± SD ( n = 10 each). Unpaired student t tests were performed (miR-183C fl/fl vs . miR-183C −/- , miR-182 fl/fl vs . miR-182 −/− ); *, p < 0.05; and **, p < 0.01.

Article Snippet: As we previously reported ( ; ; ; ), TaqMan microRNA assays (Applied Biosystems, Waltham, MA, United States) were used to quantify miR-183C miRNAs (miR-182 ID 002599; miR-183 ID 00269; miR-96 ID 000186; snoRNA202 ID 001232, Applied Biosystems) expression levels according to the manufacturer’s instruction.

Techniques:

Evaluate the effect of miR-182 and miR-183C deletion on splenic lymphocyte composition in B6/lpr mice. The different lymphocyte subsets in the freshly-isolated splenocytes were analyzed by flow cytometry. Please see for the gating of different immune cell subsets in the splenocytes. (A,B) Summary data of the frequency of CD4 + T cells, CD8 + T cells, CD19 + cells, and CD3 + B220 + CD4 − CD8 − double negative T cells (DN T cells) in the splenocytes of miR-183C −/− and control miR-183C fl/fl B6/lpr (A) , miR-182 −/− and control miR-182 fl/fl B6/lpr (B) . The graphs show means ± SD ( n ≥ 8). (C–J) Summary data of the frequency of differentiated CD4 + CD25 + Foxp3 + (C,G) , CD19 + GL7 + IgD − GCB (D,H) , CD3 + CD4 + CXCR5 + PD1 + T FH (E,I) , CD19 − CD138 + plasma (F,J) cells in the splenocytes of miR-183C −/− and miR-183C fl/fl (C–F) , miR-182 −/− and miR-182 fl/fl (G–J). The graphs show means ± SD ( n ≥ 5). Unpaired student t tests were performed (miR-183C fl/fl vs . miR-183C −/− , miR-182 fl/fl vs . miR-182 −/− ) for statistical analysis. No significant differences were observed in the difference immune cell subsets between knockout and control mice.

Journal: Frontiers in Genetics

Article Title: Deletion of microRNA-183-96-182 Cluster in Lymphocytes Suppresses Anti-DsDNA Autoantibody Production and IgG Deposition in the Kidneys in C57BL/6-Fas lpr/lpr Mice

doi: 10.3389/fgene.2022.840060

Figure Lengend Snippet: Evaluate the effect of miR-182 and miR-183C deletion on splenic lymphocyte composition in B6/lpr mice. The different lymphocyte subsets in the freshly-isolated splenocytes were analyzed by flow cytometry. Please see for the gating of different immune cell subsets in the splenocytes. (A,B) Summary data of the frequency of CD4 + T cells, CD8 + T cells, CD19 + cells, and CD3 + B220 + CD4 − CD8 − double negative T cells (DN T cells) in the splenocytes of miR-183C −/− and control miR-183C fl/fl B6/lpr (A) , miR-182 −/− and control miR-182 fl/fl B6/lpr (B) . The graphs show means ± SD ( n ≥ 8). (C–J) Summary data of the frequency of differentiated CD4 + CD25 + Foxp3 + (C,G) , CD19 + GL7 + IgD − GCB (D,H) , CD3 + CD4 + CXCR5 + PD1 + T FH (E,I) , CD19 − CD138 + plasma (F,J) cells in the splenocytes of miR-183C −/− and miR-183C fl/fl (C–F) , miR-182 −/− and miR-182 fl/fl (G–J). The graphs show means ± SD ( n ≥ 5). Unpaired student t tests were performed (miR-183C fl/fl vs . miR-183C −/− , miR-182 fl/fl vs . miR-182 −/− ) for statistical analysis. No significant differences were observed in the difference immune cell subsets between knockout and control mice.

Article Snippet: As we previously reported ( ; ; ; ), TaqMan microRNA assays (Applied Biosystems, Waltham, MA, United States) were used to quantify miR-183C miRNAs (miR-182 ID 002599; miR-183 ID 00269; miR-96 ID 000186; snoRNA202 ID 001232, Applied Biosystems) expression levels according to the manufacturer’s instruction.

Techniques: Isolation, Flow Cytometry, Knock-Out

Exam the effect of miR-182 and miR-183C deletion on IFNγ, IL-6, and IL-17 production in in vitro activated splenocytes with different stimuli. The freshly isolated splenocytes from miR-183C −/- B6/lpr (A–C) , miR-182 −/− B6/lpr (D–F) and their respective littermate control miR-miR-183C fl/fl and miR-182 fl/fl B6/lpr mice were stimulated with anti-CD3 plus anti-CD28 for 24 h, PMA plus ionomycin for 6 h, non-pathogenic Th17 stimuli or pathogenic Th17 stimuli for 72 h. The production specific cytokines in culture supernatant were measured by ELISA. (A–C) Summary graphs show deletion of miR183C in vivo in B6/lpr mice suppressed IFNγ, but not IL-6 and IL-17 in splenocytes following in vitro stimulation with specific stimuli. (D–F) Summary graphs show deletion of miR182 alone in vivo in B6/lpr mice suppressed IFNγ, but not IL-6 and IL-17 in in vitro activated splenocytes with specific stimuli. The graphs were shown as means ± SD ( n ≥ 5). Unpaired student t tests were performed (miR-183C fl/fl vs . miR-183C −/− , miR-182 fl/fl vs . miR-182 −/− ) for statistical analysis; *, p < 0.05; **, p < 0.01; and ***, p < 0.005.

Journal: Frontiers in Genetics

Article Title: Deletion of microRNA-183-96-182 Cluster in Lymphocytes Suppresses Anti-DsDNA Autoantibody Production and IgG Deposition in the Kidneys in C57BL/6-Fas lpr/lpr Mice

doi: 10.3389/fgene.2022.840060

Figure Lengend Snippet: Exam the effect of miR-182 and miR-183C deletion on IFNγ, IL-6, and IL-17 production in in vitro activated splenocytes with different stimuli. The freshly isolated splenocytes from miR-183C −/- B6/lpr (A–C) , miR-182 −/− B6/lpr (D–F) and their respective littermate control miR-miR-183C fl/fl and miR-182 fl/fl B6/lpr mice were stimulated with anti-CD3 plus anti-CD28 for 24 h, PMA plus ionomycin for 6 h, non-pathogenic Th17 stimuli or pathogenic Th17 stimuli for 72 h. The production specific cytokines in culture supernatant were measured by ELISA. (A–C) Summary graphs show deletion of miR183C in vivo in B6/lpr mice suppressed IFNγ, but not IL-6 and IL-17 in splenocytes following in vitro stimulation with specific stimuli. (D–F) Summary graphs show deletion of miR182 alone in vivo in B6/lpr mice suppressed IFNγ, but not IL-6 and IL-17 in in vitro activated splenocytes with specific stimuli. The graphs were shown as means ± SD ( n ≥ 5). Unpaired student t tests were performed (miR-183C fl/fl vs . miR-183C −/− , miR-182 fl/fl vs . miR-182 −/− ) for statistical analysis; *, p < 0.05; **, p < 0.01; and ***, p < 0.005.

Article Snippet: As we previously reported ( ; ; ; ), TaqMan microRNA assays (Applied Biosystems, Waltham, MA, United States) were used to quantify miR-183C miRNAs (miR-182 ID 002599; miR-183 ID 00269; miR-96 ID 000186; snoRNA202 ID 001232, Applied Biosystems) expression levels according to the manufacturer’s instruction.

Techniques: In Vitro, Isolation, Enzyme-linked Immunosorbent Assay, In Vivo

miR-183C and miR-182 miRNAs regulate IFNγ production via targeting Foxo1. (A) An inverse relationship between miR-183 miRNAs expression of Foxo1 protein expression in control MRL and autoimmune-prone MRL/lpr mice splenocytes. The left graph showed relative miR-183, -96, -182 miRNAs expression in the splenocytes of MRL and MRL/lpr mice (14–15 weeks of age). The graph showed means ± SD ( n = 3 each). Unpaired student t -test (MRL vs. MRL/lpr); *, p < 0.05; **, p < 0.01. The right panel showed western blotting of Foxo1 and β-actin (loading control) protein expression in MRL and MRL/lpr splenocytes. The representative western blotting image from three independent experiment was shown. (B) Increased Foxo1 protein in antagomir-182 or antagomir-183C treated splenocytes from MRL/lpr mice. The representative western blotting image from two independent experiments was shown. (C) Increased Foxo1 protein expression in splenic CD4 + T cells from miR-183C −/− B6/lpr (miR-183C −/− ), miR-182 −/− B6/lpr (miR-182 −/− ) mice when compared to the cells from their respective control miR-183C fl/fl B6/lpr (miR-183C fl/fl ) and miR-182 fl/fl B6/lpr (miR-182 fl/fl ) mice. The representative western blotting image from at least three independent experiments was shown. (D) Reduced Foxo1 mRNA expression in Foxo1 siRNA treated cells when compared to negative control siRNA (NC siRNA) treated cells. The graph showed means ± SD ( n = 2 each). (E–H) Inhibition of Foxo1 in vitro in splenocytes of miR-183 −/− (E,F) and miR-182 −/− (G,H) mice increased IFNγ, but not IL-6 production in response to anti-CD3/anti-CD28 or PMA/ionomycin stimulation. The cytokine level in Foxo1 siRNA treated samples were shown as the percentage of paired NC siRNA treated cells. The graphs were shown as means ± SD ( n ≥ 4). Paired student t tests were performed (NC siRNA vs. Foxo1 siRNA); *, p < 0.05 and **, p < 0.01.

Journal: Frontiers in Genetics

Article Title: Deletion of microRNA-183-96-182 Cluster in Lymphocytes Suppresses Anti-DsDNA Autoantibody Production and IgG Deposition in the Kidneys in C57BL/6-Fas lpr/lpr Mice

doi: 10.3389/fgene.2022.840060

Figure Lengend Snippet: miR-183C and miR-182 miRNAs regulate IFNγ production via targeting Foxo1. (A) An inverse relationship between miR-183 miRNAs expression of Foxo1 protein expression in control MRL and autoimmune-prone MRL/lpr mice splenocytes. The left graph showed relative miR-183, -96, -182 miRNAs expression in the splenocytes of MRL and MRL/lpr mice (14–15 weeks of age). The graph showed means ± SD ( n = 3 each). Unpaired student t -test (MRL vs. MRL/lpr); *, p < 0.05; **, p < 0.01. The right panel showed western blotting of Foxo1 and β-actin (loading control) protein expression in MRL and MRL/lpr splenocytes. The representative western blotting image from three independent experiment was shown. (B) Increased Foxo1 protein in antagomir-182 or antagomir-183C treated splenocytes from MRL/lpr mice. The representative western blotting image from two independent experiments was shown. (C) Increased Foxo1 protein expression in splenic CD4 + T cells from miR-183C −/− B6/lpr (miR-183C −/− ), miR-182 −/− B6/lpr (miR-182 −/− ) mice when compared to the cells from their respective control miR-183C fl/fl B6/lpr (miR-183C fl/fl ) and miR-182 fl/fl B6/lpr (miR-182 fl/fl ) mice. The representative western blotting image from at least three independent experiments was shown. (D) Reduced Foxo1 mRNA expression in Foxo1 siRNA treated cells when compared to negative control siRNA (NC siRNA) treated cells. The graph showed means ± SD ( n = 2 each). (E–H) Inhibition of Foxo1 in vitro in splenocytes of miR-183 −/− (E,F) and miR-182 −/− (G,H) mice increased IFNγ, but not IL-6 production in response to anti-CD3/anti-CD28 or PMA/ionomycin stimulation. The cytokine level in Foxo1 siRNA treated samples were shown as the percentage of paired NC siRNA treated cells. The graphs were shown as means ± SD ( n ≥ 4). Paired student t tests were performed (NC siRNA vs. Foxo1 siRNA); *, p < 0.05 and **, p < 0.01.

Article Snippet: As we previously reported ( ; ; ; ), TaqMan microRNA assays (Applied Biosystems, Waltham, MA, United States) were used to quantify miR-183C miRNAs (miR-182 ID 002599; miR-183 ID 00269; miR-96 ID 000186; snoRNA202 ID 001232, Applied Biosystems) expression levels according to the manufacturer’s instruction.

Techniques: Expressing, Western Blot, Negative Control, Inhibition, In Vitro